Living Image 3.1

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9. Generating a Surface Topography 126 5. Click Next to display the mask. The mask is a purple overlay on the subject image that defines the area of interest for the surface topography reconstruction. The mask should match the underlying photograph of the subject as closely as possible without including any area outside the subject image. 6. If you want to smooth the surface, confirm the default surface generation options and surface smoothing parameters or enter new values. (For more details on the parameters, see Table 9.1). 7. If you want to save the results, confirm the default name for the results or enter a new name. 8. If necessary, adjust the threshold value so that the mask fits the subject image as closely as possible without including any area outside of the subject. To change the threshold, do one of the following: Press the left or right arrow keys on the keyboard. Move the Threshold slider left or right. Click the arrows or enter a new value in the box. 9. Click Finish. - The surface and 3D tools appear. For more details on the tools, see page 150. f Surface
<strong>Living</strong> <strong>Image</strong> ® Software User’s Manual Table 9.1 Surface topography options & parameters Item Description Surface Reconstruction Structured Light A drop-down list of structured light images in the sequence. Select one for use in reconstructing the surface. SL Binning The binning level applied to the pixels that are used to construct the surface topography from the structured light image. If Auto is chosen, the software automatically determines the appropriate binning level. The higher the binning level, the lower the mesh resolution. For more details on binning, see page 192. Reconstruct Click to generate the surface. Surface Smoothing Smoothing Level The amount of smoothing to apply to a reconstructed surface. Restore Removes smoothing that was applied to a surface. Loss Recovery Smoothing can cause loss in the surface volume or height. Make a selection from the drop-down list to reduce losses. 'Height' is recommended for IVIS 200 or IVIS Spectrum surfaces. Smooth Initiates the smoothing specified. Save Results Name The name of the surface. Delete Click to delete the surface selected from the Name dropdown list. Load Click to load the surface selected from the Name dropdown list. Save Click to save the surface to the name entered in the Name drop-down list. 127
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Living Image 3.1

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