Living Image 3.1

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2. Getting Started Table 2.2 Image analysis tools Tool Palette Use The Tools to... See Page Image Adjust Tune the photograph brightness, gamma (similar to contrast), or opacity Set the image display color scale min and max Select a color table for image display 52 8 Corrections/Filtering Subtract dark background from the image data Apply flat field correction to the image data Specify pixel binning Smooth the pixel signal Image Information Display x,y coordinates and intensity data at a user-selected location on the image Display a histogram of pixel intensities in an image Plot the intensity (y-axis) at each pixel (x-axis) along a user-specified line in the image Measure distance in an image ROI Measure counts or photons in a user-specified region of interest (ROI) and compute measurement statistics (for example, average, min, max, standard deviation) Measure efficiency in the ROI and compute measurement statistics (for fluorescent images only) Table 2.3 Analyzing image sequences Analysis Description Wizard Setup See Page Planar Spectral Image Analysis Determines the average depth and total photon flux of a bioluminescent point source in a user-specified region of interest. Analyzes a sequence of bioluminescent images acquired using different emission filters. Image Math A method for mathematically combining two images (add, multiply, or subtract). Use image math to: Remove autofluorescence from a fluorescent image Display multiple bioluminescent or fluorescent images on the same photographic image so that you can view multiple reporters in one image Spectral Unmixing Removes tissue autofluorescence from a fluorescence image. Analyzes a sequence of fluorescence images acquired using the fluorophore excitation filter and several different emission filters. Spectral unmixing can be applied to images acquired using epiillumination (excitation light above the stage) or transillumination (excitation light below the stage). Point Source Fitting Estimates the optical properties of tissue, the location and power of a point source, or the fluorescent yield of fluorophores. 3D Fluorescence Imaging Tomography (FLIT) 3D Diffuse Luminescence Tomography (DLIT) A 3-dimensional reconstruction of the image (tomographic analysis) that estimates the location and intensity of a fluorescent lightemitting source. Analyzes a sequence of fluorescent images acquired on the IVIS Spectrum at different transillumination points (excitation light below the stage). A 3-dimensional reconstruction of the subject (tomographic analysis) that estimates the depth and intensity of a bioluminescent lightemitting source. 55 56 69 Yes 107 No 104 Yes 113 Yes 129 Yes 138 Yes 142
Living Image ® Software User’s Manual 2.2 Starting the Living Image ® Software For information on installing the software, see the Installation Guide included on the Living Image CD ROM. By default, the software is installed at: PC: C:Programs:Xenogen:Living Image 3.1 Macintosh: Applications:Xenogen:LivingImage 3.1 NOTE All components of the IVIS ® Imaging System should be left on at all times due to the long cooling time required to reach operating (demand) temperature. It is also important to leave the system on to enable automatic overnight electronic background measurements. Periodically rebooting the computer is permissible and does not affect the camera operation. To start the software: 1. PC Users: Click the Windows Start menu button and select All Programs → Living Image. Alternatively, click the Living Image ® software desktop icon . Macintosh Users: Click the Living Image software desktop icon or run the software from the application folder. - The main window appears. 2. Select a user ID from the dropdown list or enter a new User ID (up to three letters), and click OK. - The control panel appears if the workstation controls the IVIS Imaging System (see next page). 9
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Living Image 3.1

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