Living Image 3.1

4. Working With Images
46
Table 4.3 Image window
Item Description
Units Choose counts or photons from the drop-down list for the image data.
For more details on counts and photons, see Appendix D, page 199.
Display To choose an image display mode in the image window, make a
selection from the Display drop-down list. See Figure 4.5 for examples
of the display modes.
Overlay A pseudocolor image of luminescent or fluorescent image data displayed
over a grayscale photographic image.
Photograph A grayscale image that is captured when the IVIS ® Imaging System
illumination lights are activated.
Luminescent A pseudocolor image of the luminescent or fluorescent data captured
during an exposure when the IVIS Imaging System illumination lights are
off.
Fluorescent A pseudocolor image of the fluorescent data captured during an
exposure when the IVIS Imaging System illumination lights are off.
Background The CCD camera background acquired with the camera shutter closed.
(See Appendix E, page 203.)
Bias An electronic offset that exists on every pixel. This means that the zero
photon level in the readout is not actually zero, but is typically a few
hundred counts per pixel. The read bias offset is reproducible within
errors defined by the read noise, another quantity that must be
determined for quantitative image analysis.
Saturation Map Displays image regions that saturated the CCD digitizer (>65535 counts)
in red. ROI measurements should not be made on saturated regions.
ROI measurements made on image regions that do not contain
saturated pixels are accurate (unless the image is badly saturated).
Structure A structured light image of parallel laser lines scanned across the
subject. (Available in the IVIS ® Imaging System 200 Series and IVIS
Spectrum.) The surface topography of the subject is determined from
the structured light image.
Reference A structured light image of a white plate that is acquired and stored on
disk prior to instrument installation.
3D View A three-dimensional rendering of the subject. For more details see
Appendix H, page 233.
Export Opens the Export Active View As Image box so that the active image
data can be exported (bmp, jpg, png, tiff, or postscript format).
Info Click to display or hide information about the image in the image
window.