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Living Image 3.1

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<strong>Living</strong> <strong>Image</strong> ® Software User’s Manual<br />

4. In the <strong>Image</strong> Math window that appears, select the primary image in box A. Select<br />

the background image in box B.<br />

Note: For more details on items in the <strong>Image</strong> Math window, see Table 6.1, page 100.<br />

5. Select the math function 'A-B*k' in the Result drop-down list.<br />

6. Click and select the ROI (created in step 2) from the drop-down list.<br />

- The background-corrected signal is displayed.<br />

7. To view the mathematical result (overlay mode) in a separate image window, click<br />

Display Result For Measuring.<br />

Note: If necessary, use the Color Scale Min and Max sliders in the <strong>Image</strong> Adjust<br />

tools to adjust the image display.<br />

To save the new image:<br />

1. Click the Save button . Alternately, select File → Save on the menu bar.<br />

2. In the dialog box that appears, select a directory, and click Save.<br />

- A folder of data is saved to the selected location (AnalyzedClickInfo.txt,<br />

ClickInfo.txt, luminescent and photographic TIF images).<br />

To export the new image to a graphic file:<br />

1. Click the Export button .<br />

2. In the dialog box that appears, select a directory, enter a file name, and select the<br />

file type from the Save as type drop-down list.<br />

3. Click Save.<br />

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