Living Image 3.1

6. Image Math
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To save the new image:
1. Click the Save button . Alternatively, select File → Save on the menu bar.
2. In the dialog box that appears, select a directory, and click Save.
- A folder of data is saved to the selected location (AnalyzedClickInfo.txt,
ClickInfo.txt, luminescent and photographic TIF images).
To export the image to a graphic file:
1. Click the Export button .
2. In the dialog box that appears, select a directory, enter a file name, and select the
file type from the Save as type drop-down list.
3. Click Save.
Table 6.1 Image Math window
Item Description
Color Ranges for A and B
Full Choose this option to set the Max and Min values to the
maximum and minimum data values in the image.
Auto When this option is chosen, the software sets the Min and
Max values to optimize image display and suppress
background noise. The Min and Max settings can be manually
adjusted to further optimize the image display for your needs.
Note: The color bar scale does not affect the image math
result.
Color Ranges for Result
Image
Full See above.
Auto See above.
Min = 0 Choose this option to set the minimum data value to zero.
Results Drop-down list of mathematical functions that can be used to
generate the new image, including:
A - B*k
A + B*k
A * B*k
A/B if Counts(B)>k (Useful for fluorescence tomography.)
k, Image Math window A user-specified scaling factor applied in the results function.
k, Fluorescent Background The software computes k = the ratio of the autofluorescent
Subtraction window
signal measured using the background filter to the
autofluorescent signal measured using the excitation filter in a
region on the animal where no fluorophore is present.
Compute ‘k’ from ROI This option is useful for subtracting fluorescence background.
Draw the same ROI in both images on an area considered
background. In the “Compute ‘k’ from ROI” drop-down list,
select the same ROI in each image.
with Photo from Choose this option to display the new image in overlay mode
using the selected photographic image. (This option is only
available if one of the selected images is an overlay.