Living Image 3.1

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11. 3D Reconstruction of Sources 174 appears. To copy all results: 1. In the Results tab, right-click the results table and select Copy All from the shortcut menu that appears. - All of the results table is copied to the system clipboard. To export results: 1. In the results tab, right-click the results table and select Export Results from the shortcut menu that appears. 2. In the dialog box that appears, choose a folder for the results, enter a file name, and click Save.
Living Image ® Software User’s Manual 12 Troubleshooting Guide 12.1 DLIT/FLIT Analysis Troubleshooting Table 12.1 DLIT/FLIT analysis Issue Solution Large voxels (> 2 mm) The Ndata>Nvoxels is required for an overdetermined problem. It is possible that there are not enough data (N Surface). This can occur because it is desired that all wavelengths (DLIT) and all trans-illumination source positions (FLIT) are represented in the data equally. In the DLIT case, if the signal in a short wavelength is very low, then the sampled data can be too few for sufficient refinement of the voxel gridding. – To increase the data to be included, determine the [photons/sec/cm2/sec] level for ~30 counts on the low signal image (this is the level of noise) using Image Adjust in the Tool Palette. Enter in the [photons/sec/cm2/sec] value into the 'Minimum Radiance' column next to the wavelength/image number in the 'Analyze Tab' in the DLIT/FLIT 3D Reconstruction menu of the Tool Palette. It is best to include the low signal data as it adds constraining information to the solution. Note: Using the Imaging Wizard should reduce the occurrence of low signal images in the data sequences. No sources in solution In DLIT or FLIT, this can occur if the surface is not correct. That is, if a surface is imported into the 3D View from another source other than from the Structured Light Analysis. In FLIT, if you choose an emission Source Spectrum in the Properties Tab (an Advanced User feature) which is incorrect for the fluorophore of interest, this can also result in no sources. In FLIT, if there is very little signal detected at the surface and the 'Background' box is checked in the Params tab, then it is possible that only the background fluorescence is simulated. Surfaces are spiky The most common source of spiky surfaces are folds in the animal skin or animal fur, which corrupt the desired smooth lines projected on the animal from the laser galvanometer. – Adjusted the 'Path Averaging Size' in the 'Surface Topography' menu in the Tool Palette can help reconstruct an improved surface – Smoothing the surface by using the 'Smooth' feature in the tool palette Surface Topography menu can also help improve the surface. 175
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Living Image 3.1

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