Living Image 3.1

More documents

Recommendations

Info

5. Working With ROI Tools Table 5.1 Types of ROIs Description Available ROI Drawing Methods Available Shapes 70 NOTE Type of ROI Measurement ROI Average Background ROI Subject ROI Measures the signal intensity in an area of an image. Manual Automatic Free draw Circle, square, grid, or contour Measures the average signal intensity in a user-specified area of the image that is considered background. Note: Using this type of ROI is optional. If the animal has significant autoluminescence or autofluorescence, you can determine a backgroundcorrected signal in a measurement ROI by subtracting an average background ROI from a measurement ROI. Manual Free draw For a quick guide to drawing a measurement ROI, see page 73. Identifies a subject animal in an image. Note: Using this type of ROI is optional. It provides a convenient way to automatically associate (link) a measurement and average background ROI for backgroundcorrected ROI measurements when there is significant autoluminescence or autofluorescence. Manual Automatic Free draw Circle or square Square
<strong>Living</strong> <strong>Image</strong> ® Software User’s Manual 5.2 ROI Tools To display the ROI tools: 1. Open an image or image sequence and click ROI Tools in the tool palette. ROI tools Table 5.2 Tool palette, ROI tools Item Description Click to select the number of circle ROIs to add to the active image. Apply to Sequence Click to select the number of square ROIs to add to the active image. Click to specify the grid pattern for a measurement ROI that you want to add to the active image. This tool is useful for an image of a multi-well culture plate or microtiter plate. Click and select Auto All to automatically draw ROIs in the image using the auto ROI parameters. Click and select Auto 1 to automatically draw one ROI at a user-selected location using the auto ROI parameters. For more details on using the auto ROI features, see page 76. Click to display the ROI Measurements table or compute intensity signal in an ROI. Click to display a drop-down list of options to delete an ROI(s) in the active image. For more details, see page 90. Note: These commands do not delete the ROIs that are saved to the system (listed in the Menu Name drop-down list). Choose this option to apply the selected ROI to all images in a sequence. 71
Page 1 and 2:
Living Image® Software User’s Ma
Page 3 and 4:
Living Image ® Software User’s M
Page 5 and 6:
Page 7 and 8:
Page 9 and 10:
Page 11 and 12:
Page 13 and 14:
Page 15 and 16:
Page 17 and 18:
Page 19 and 20:
Page 21 and 22:
Page 23 and 24:
Page 25 and 26: Living Image ® Software User’s M
Page 75: Living Image ® Software User’s M
Page 127 and 128:
Page 129 and 130:
Page 131 and 132:
Page 133 and 134:
Page 135 and 136:
Page 137 and 138:
Page 139 and 140:
Page 141 and 142:
Page 143 and 144:
Page 145 and 146:
Page 147 and 148:
Page 149 and 150:
Page 151 and 152:
Page 153 and 154:
Page 155 and 156:
Page 157 and 158:
Page 159 and 160:
Page 161 and 162:
Page 163 and 164:
Page 165 and 166:
Page 167 and 168:
Page 169 and 170:
Page 171 and 172:
Page 173 and 174:
Page 175 and 176:
Page 177 and 178:
Page 179 and 180:
Page 181 and 182:
Page 183 and 184:
Page 185 and 186:
Page 187 and 188:
Page 189 and 190:
Page 191 and 192:
Page 193 and 194:
Page 195 and 196:
Page 197 and 198:
Page 199 and 200:
Page 201 and 202:
Page 203 and 204:
Page 205 and 206:
Page 207 and 208:
Page 209 and 210:
Page 211 and 212:
Page 213 and 214:
Page 215 and 216:
Page 217 and 218:
Page 219 and 220:
Page 221 and 222:
Page 223 and 224:
Page 225 and 226:
Page 227 and 228:
Page 229 and 230:
Page 231 and 232:
Page 233 and 234:
Page 235 and 236:
Page 237 and 238:
Page 239 and 240:
Page 241 and 242:
Page 243 and 244:
Page 245 and 246:
Page 247 and 248:
Page 249 and 250:
Page 251 and 252:
Page 253 and 254:
Page 255 and 256:
Page 257 and 258:
show all

Living Image 3.1

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?