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Living Image 3.1

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<strong>Living</strong> <strong>Image</strong> ® Software User’s Manual<br />

5.2 ROI Tools<br />

To display the ROI tools:<br />

1. Open an image or image sequence and click ROI Tools in the tool palette.<br />

ROI<br />

tools<br />

Table 5.2 Tool palette, ROI tools<br />

Item Description<br />

Click to select the number of circle ROIs to add to the active image.<br />

Apply to<br />

Sequence<br />

Click to select the number of square ROIs to add to the active image.<br />

Click to specify the grid pattern for a measurement ROI that you want to add<br />

to the active image. This tool is useful for an image of a multi-well culture<br />

plate or microtiter plate.<br />

Click and select Auto All to automatically draw ROIs in the image using the<br />

auto ROI parameters. Click and select Auto 1 to automatically draw one ROI<br />

at a user-selected location using the auto ROI parameters. For more details<br />

on using the auto ROI features, see page 76.<br />

Click to display the ROI Measurements table or compute intensity signal in an<br />

ROI.<br />

Click to display a drop-down list of options to delete an ROI(s) in the active<br />

image. For more details, see page 90.<br />

Note: These commands do not delete the ROIs that are saved to the system<br />

(listed in the Menu Name drop-down list).<br />

Choose this option to apply the selected ROI to all images in a sequence.<br />

71

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