Living Image 3.1

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5. Working With ROI Tools 5.4 Measurement ROIs 74 The ROI Measurements table displays data for all ROIs created during a session (one ROI per row). The table provides a convenient way to review and export ROI data. For more details on the table, see Managing the ROI Measurements Table, page 91. To obtain the intensity signal in a user-specified area of an image, draw a measurement ROI on the image. There are three ways to draw measurement ROIs: Drawing Method Description See Page Manual Places one or more ROIs (circular, square, or grid shape) on the image. 75 Automatic The software automatically locates and draws a contour ROI(s) on the image. To do this, the software locates the peak pixel intensities in the image and searches the neighborhood around a peak pixel. A pixel is included in the ROI if the pixel intensity is greater than the threshold%, a user-specified percentage of the peak pixel intensity. 76 Free draw Draw line segments that define the ROI. 78
<strong>Living</strong> <strong>Image</strong> ® Software User’s Manual Manually Drawing a Measurement ROI 1. Open an image or image sequence, and in the ROI tools, select Measurement ROI from the Type drop-down list. 2. To specify the ROI shape: a. Click the Circle , Square , or Grid button . The grid shape is useful for drawing a grid of ROIs on an image of a microplate plate. b. On the drop-down list that appears, select the number of ROIs that you want to add to the image or the grid ROI dimensions. - The ROI(s) and intensity label(s) appear on the image. If you are working with a sequence, open an image to show the ROI intensity. 3. Adjust the ROI position: a. Place the mouse pointer over the ROI. When the pointer becomes a , click the ROI. b. Drag the ROI. 4. Adjust the ROI dimensions: a. Place the mouse pointer over the ROI. When the pointer becomes a ROI. , click the b. Place the mouse pointer over an ROI handle to resize the ROI. so that it becomes a . Drag the handle Note: You can also change the ROI position or size using the adjustment controls in the ROI Properties box (see Moving an ROI, page 85 and Editing ROI Dimensions, page 86). 75
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Living Image 3.1

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