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Living Image 3.1

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<strong>Living</strong> <strong>Image</strong> ® Software User’s Manual<br />

Table 4.10 Crop box position & dimensions<br />

Item Description<br />

4.10 Rendering Intensity Data in Color<br />

The colorize tool renders luminescence or fluorescence data in color, enabling you to<br />

see both intensity and spectral information in a single view. The tool provides a useful<br />

way to visualize multiple probes or scale probe signals that are not in the visible range.<br />

NOTE<br />

x,y coordinates at the upper left corner of the box.<br />

x,y coordinates of lower right corner of the box.<br />

Box width and height.<br />

Distance Length of the diagonal from the upper left to lower right corner of the<br />

box.<br />

The colorize tool is only available if Show Advanced Options is selected in the general<br />

preferences (see page 182).<br />

To view colorized intensity data:<br />

1. Open an image sequence.<br />

In this example, images<br />

were acquired using<br />

different combinations of<br />

excitation and emission<br />

filters. The samples are<br />

quantum dot nanocrystals<br />

(700 or 800 nm).<br />

2. Select Tools → Colorize “sequence name”_SEQ on the menu bar.<br />

- The software renders each luminescent or fluorescent image in color and<br />

combines them into a single image.<br />

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