Living Image 3.1

11. 3D Reconstruction of Sources
11.3 DLIT & FLIT Results
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The Results tab displays information about the photon density, voxels, and DLIT or
FLIT algorithm parameters.
Figure 11.2 3D reconstruction results, FLIT (left) and DLIT (right)
NOTE
For more details on the DLIT and FLIT algorithm parameters, see Appendix H,
page 233. Sometimes adjusting the DLIT algorithm parameters improves the fit of the
simulated photon density to the measured photon density data.
Table 11.3 3D reconstruction results
Item Description
Optimized fit parameters
Total source flux (phot/s) (DLIT The sum of the bioluminescent source intensities.
result)
Total fluorescence yield (N mm2 ) The total sum of the fluorescent yield. The quantity
(FLIT result)
measured is:
(Fluorescence quantum efficiency for the excitation
wavelength to emission wavelength photons)*(Excitation
wavelength photon absorption cross section)*(Fluorophore
number density)*(Volume of voxel size). To convert the
'Fluorescence Yield' measurement to the number of
fluorescent compounds, divide the 'Fluorescent Yield'
values by measured quantities of [Fluorescence quantum
efficiency of excitation wavelength conversion to emission
wavelength]*[Excitation photon absorption cross section]
for the fluorophore.