Living Image 3.1
Living Image 3.1
Living Image 3.1
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<strong>Living</strong> <strong>Image</strong> ® Software User’s Manual<br />
Table 10.1 Tool palette, point source fitting<br />
Item<br />
Analysis Tab<br />
Description<br />
<strong>Image</strong> # <strong>Image</strong> number in the active sequence.<br />
ExWL Excitation wavelength<br />
EmWL Emission wavelength<br />
MinRadiance Minimum surface radiance used for model fitting.<br />
x,y<br />
Params Tab<br />
x- and y-coordinates of the bottom illumination source.<br />
Model Type The image acquisition mode.<br />
Angle Limit (deg) The angle limit refers to the angle between the object surface<br />
normal and the optical axis. (For more details, see page 240.)<br />
Spatial Filter Filters out the noisy data at the mouse edges. A setting of 0.1<br />
means that the analysis includes 90% of the data from the<br />
center of mass to the edges.<br />
Parameter starting values Note: Selecting a tissue Properties tab automatically updates<br />
MuaEm, MusEm/ MuaEx, and MusEx in the Params tab.<br />
x, y, or z Source coordinates.<br />
F-yield/Power Fluorescence yield/strength of illumination or bioluminescence<br />
source.<br />
MuaEm Absorption coefficient at the emission wavelength.<br />
MusEm Reduced scattering coefficient at the emission wavelength.<br />
MuaEx Absorption coefficient at the excitation wavelength.<br />
MusEx Reduced scattering coefficient at the excitation wavelength.<br />
Restore Defaults Resets the model type, algorithm starting parameters and<br />
algorithm options to the default values.<br />
Mask A drop-down list of ROIs in the selected image. Select an ROI<br />
to compute only the source in the ROI.<br />
Statistics Weighting Choose this option to apply a statistical weighting technique to<br />
help reduce the error associated with high radiance<br />
measurements.<br />
LM Fitting<br />
Properties Tab<br />
Click to begin the point source fitting.<br />
Tissue Properties Make a selection from this drop-down list to specify starting<br />
values for the parameters other than the defaults. Note:<br />
Selecting a tissue property automatically updates MuaEm,<br />
MusEm/ MuaEx, and MusEx in the Params tab.<br />
Internal medium index of The internal medium index of the tissue selected from the<br />
refraction<br />
Results Tab<br />
Tissue Properties drop-down list. You can also enter a userspecified<br />
value.<br />
MuaEm Absorption coefficient at the emission wavelength.<br />
MusEx Reduced scattering coefficient at the excitation wavelength.<br />
Mueff Effective attenuation coefficient<br />
Mueff = 3Mua( Mua + Mus)<br />
Diff Diffusion coefficient, Diff = (Mua +Mus)/3<br />
131
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