Living Image 3.1

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Index adjusting the lens aperture 191 exposure time 192 field of view 192 diffusion model 226 display modes 46 display units counts 199 efficiency 201 photons 200 DLIT troubleshooting 175 DLIT results 144–146 manage 173 drift correction 203 E edit image sequence 28–30 edit image sequence 42–43 efficiency 201, 211, 214–215 electronic background dark charge 204 dark current 203 drift 203 read bias 203 export image data 34 exposure time 192 F f/stop fluorescent imaging 214 field of view 192 filter bandpass 211 fluorescent 212 filter spectra 211 flat field correction 55 flat fielding 202 FLIT troubleshooting 175 FLIT results 144–146 manage 173 fluorescence adaptive background subtraction 221 reconstruct 3D sources 142–143 fluorescence efficiency 214–215 fluorescence image (epi-illumination) 17–18 248 fluorescence image (trans-illumination) 19–21 fluorescence imaging components 209–211 fluorescent filters 212 fluorescent imaging efficiency 211 f/stop 214 normalization 211 focus manually 32–33 FOV settings 180 G graphic image data 197 H High Reflectance Hemisphere 205 histogram 59 I image adjusting appearance 52 cascade 48 correct or filter 55–56 correcting/filtering tools 55–56 histogram 59 image window 45–46 information 49 label 51 line profile 60 magnify or pan 54 measurements 61 pixel data 58 tag 48 thumbnails 40 tile 48 image analysis tools 8 image data browse 35–37 colorize 63–64 export 34 open 37–39 save manually 33 image information 56–57 image layout window 66–67 image math 98–101 image overlay tool 104–106 image sequence 40–43 acquire 30–31
Living Image ® Software User’s Manual analyses 8 application-specific 22 create from individual images 43–44 edit 28–30, 42–43 imaging wizard 23–25 manual setup 25–27 image window 16 3D perspective 155 display modes 46 image window (single image) 45–46 imaging & image analysis example workflow 7 overview 5–8 imaging wizard 23–25 index of refraction 145 information about an image 49 initialization See system initialization. instrument fluorescent background background instrument fluorescent background 221 IVIS Imaging System fluorescence imaging components 209–211 K kappa 145 kappa limits 145, 240 L lens aperture 191 line profile 60 Living Image browser 36 Living Image software help 10 starting 9 luciferase spectrum 227 luminescence reconstruct 3D sources 138–141 M magnify image 54 manual focusing 32–33 save data 33 manual conventions 2 measurement ROI automatically draw 76–77 measurement ROIs 70, 74–77 free draw 78 measurements 61 menu commands 243 mesh drawing style 153 lighting style 153 miscellaneous material autofluorescence 217–219 multiple reporters per photograph 104–106 N N surface limits 145, 240 NNLS optimization 145, 241 weighted fit 145, 241 normalization 211 O open image data 37–39 optical density 212 optical properties for planar spectral imaging 227 organ atlas 167 organs 162–166 overlay 198 overlaying images 104–106 overview imaging & image analysis 5–8 P pan image 54 PCA biplot 122 PCA explained variance 123 photon density 146 photon density map measured 146 simulated 146 photon radiance 200 photons 200 pixel 197 pixel data 58 planar spectral analysis optimizing precision 231 planar spectral image analysis 107–109 planar spectral imaging 225–231 diffusion model 226 intensity graph 111 linear fit graph 111 249
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Living Image 3.1

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