Living Image 3.1

More documents

Recommendations

Info

A. IVIS Acquisition Control Panel Table A.2 Additional IVIS ® System Controls for the IVIS Imaging System 200 Series or IVIS Spectrum Item Description Alignment grid Choose this option to activate a laser-generated alignment grid on the stage when the imaging chamber door is opened. The alignment grid is set to the size of the selected FOV. The grid automatically turns off after two minutes. If subject alignment is not completed in two minutes, place a check mark next to Enable Alignment Grid to turn on the grid. Note: The horizontal cross hair of the alignment grid is offset appropriately to take into account the height entered in the Subject height box. Focus Scan Mid Image - Choose this option in the Focus drop-down list to set the focal plane at the maximum dorso-ventral height of the subject at the middle of the animal. This focusing method uses the laser to scan horizontally across the middle of the subject to determine the maximum subject height along this line. This option is well suited for animal imaging because the peak height is clearly identified as the maximum height on the dorsal side along the mid-plane of the animal. Note: This focusing method is not recommended for microplates or when using a high magnification field of view (FOV A = 4.0 cm). In these situations, Manual or Subject Size focus methods are recommended. Transillumination Setup Choose this option to display the transillumination setup window that enables you to select the (IVIS Spectrum only) locations for image acquisition using bottom illumination that originates beneath the stage. 180 Table A.3 Typical field of view (FOV) settings FOV Setting IVIS Imaging System Lumina 100 Series FOV (cm) 200 Series A 4 10 3.9 B 7 15 6.5 C 10 20 13 D 12 25 19.5 E 26
Living Image ® Software User’s Manual Appendix B Preferences General Preferences . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 182 User Preferences . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 184 Acquisition . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 185 Theme . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 186 Tissue Properties . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 187 3D Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 188 You can manage user IDs and specify defaults for some parameters that are associated with the user ID selected at the start of a new session. 1. After you log on, to view the user-modifiable preferences, select Edit ➞Preferences on the menu bar. - The Preferences box appears. NOTE Any changes made to the Preferences are implemented at the start of the next session. The Acquisition tab is only available in the Living Image software that controls the IVIS ® Imaging System. 181
Page 1 and 2:
Living Image® Software User’s Ma
Page 3 and 4:
Living Image ® Software User’s M
Page 5 and 6:
Page 7 and 8:
Page 9 and 10:
Page 11 and 12:
Page 13 and 14:
Page 15 and 16:
Page 17 and 18:
Page 19 and 20:
Page 21 and 22:
Page 23 and 24:
Page 25 and 26:
Page 27 and 28:
Page 29 and 30:
Page 31 and 32:
Page 33 and 34:
Page 35 and 36:
Page 37 and 38:
Page 39 and 40:
Page 41 and 42:
Page 43 and 44:
Page 45 and 46:
Page 47 and 48:
Page 49 and 50:
Page 51 and 52:
Page 53 and 54:
Page 55 and 56:
Page 57 and 58:
Page 59 and 60:
Page 61 and 62:
Page 63 and 64:
Page 65 and 66:
Page 67 and 68:
Page 69 and 70:
Page 71 and 72:
Page 73 and 74:
Page 75 and 76:
Page 77 and 78:
Page 79 and 80:
Page 81 and 82:
Page 83 and 84:
Page 85 and 86:
Page 87 and 88:
Page 89 and 90:
Page 91 and 92:
Page 93 and 94:
Page 95 and 96:
Page 97 and 98:
Page 99 and 100:
Page 101 and 102:
Page 103 and 104:
Page 105 and 106:
Page 107 and 108:
Page 109 and 110:
Page 111 and 112:
Page 113 and 114:
Page 115 and 116:
Page 117 and 118:
Page 119 and 120:
Page 121 and 122:
Page 123 and 124:
Page 125 and 126:
Page 127 and 128:
Page 129 and 130:
Page 131 and 132:
Page 133 and 134:
Page 135 and 136: Living Image ® Software User’s M
Page 185: Living Image ® Software User’s M
Page 237 and 238:
Page 239 and 240:
Page 241 and 242:
Page 243 and 244:
Page 245 and 246:
Page 247 and 248:
Page 249 and 250:
Page 251 and 252:
Page 253 and 254:
Page 255 and 256:
Page 257 and 258:
show all

Living Image 3.1

Create successful ePaper yourself

Delete template?

Save as template?