Living Image 3.1

5. Working With ROI Tools
72
Table 5.2 Tool palette, ROI tools
Item Description
Type Choose the ROI type from the drop-down list:
Measurement - Measures the signal intensity in an area of an image.
Average Bkg - Measures the average signal intensity in a user-specified area
of the image that is considered background.
Subject ROI - Identifies a subject animal in an image. The software
automatically associated a measurement and average bkg ROI included in the
same subject ROI. Using this type of ROI is optional.
Save ROIs
Name The name of the selected ROI set or the default name for a new ROI set.
Delete Deletes the selected ROI set from the system. Note: This permanently
removes the ROI from the system.
Load Applies the ROI set selected from the Name drop-down list to the active
image.
Save Saves the ROI set in the active image.
Note: This is a global save (the ROI is saved to the system) and the ROI set
can be loaded onto any image. If you use the File ➞ Save commands to save
an image that includes an ROI, the ROI is saved with the image only (not a
global save) and is not available for loading onto other images. For more
details, see Saving ROIs, page 89.
Auto ROI Parameters that specify how the auto ROI tool draws an ROI. Note: These
Parameters are advanced options that are only available if “Show Advanced Options” is
selected in the general preferences.
Threshold % If the Auto All or Auto 1 method is selected, the Threshold % specifies the
minimum per cent of peak pixel intensity that a pixel must have to be included
in an ROI identified by the software.
Note: After ROIs are drawn on an image, if you modify the Threshold% (move
the slider or enter a new value) the software automatically updates the ROIs.
Lower Specifies a multiple (1 to 10) of the color bar minimum that sets the lower
Limit threshold for identifying an ROI. For example, if the lower limit = 2 and the
color bar minimum = 1000 counts, then the auto ROI tool will only draw an
ROI on areas of 2000 counts or greater. This helps create ROIs only in the
visible range.
Minimum Sets the minimum size of an ROI (measured in pixels). For example if the
Size minimum size is set at 50, then ROIs created on the image must be greater
than 50 pixels in size.
Preview If this option is chosen, the software draws the ROI each time a parameter is
changed. ROI parameters can be saved without drawing the ROI.
Use Bkg Choose this option to measure background-corrected signal. For more details,
Offset see page 79.
Replace If this option is chosen, all auto ROIs are replaced when new ROI(s) are
ROIs created.
Restore Restores the factory-set defaults for the auto ROI parameters.
Defaults
Save/Load Click to display or hide the tools that enable you to save, load, or delete auto
ROIs in the active data. Note: The save function saves parameters, the not
actual ROIs. This means that when you load saved auto ROI parameters, the
software draws a new ROI using the saved values (Threshold%, Lower Limit,
Minimum Size).