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Living Image 3.1

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<strong>Living</strong> <strong>Image</strong> ® Software User’s Manual<br />

NOTE<br />

The items in the IVIS System control panel depend on the particular IVIS Imaging<br />

System and the imaging mode selected (luminescent or fluorescent, <strong>Image</strong> Setup or<br />

Sequence Setup mode). For more details on<br />

3.3 About the IVIS Acquisition Control Panel & Auto Exposure Feature<br />

The control panel (Figure 3.2) provides the image acquisition functions. For details on<br />

the imaging parameters in the control panel, see Appendix A, page 177.<br />

The auto exposure setting is useful in situations where the signal strength is unknown or<br />

varies widely, for example during a time course study. When you choose auto exposure<br />

(Figure 3.2), the system acquires an image at maximum sensitivity, then calculates the<br />

required settings to achieve, as closely as possible, an image with a user-specified target<br />

max count. If the resulting image has too little signal or saturated pixels, the software<br />

adjusts the parameters and takes another image.<br />

In most cases, the default auto exposure settings provide a good bioluminescence or<br />

fluorescence image. However, you can modify the auto exposure preferences to meet<br />

your needs. For more details, see page 185.<br />

Luminescence<br />

imaging settings<br />

Fluorescence<br />

imaging settings<br />

Photographic<br />

imaging settings<br />

Structured light<br />

imaging settings<br />

Figure 3.2 IVIS acquisition control panel, auto exposure selected<br />

NOTE<br />

To acquire an image using auto exposure,<br />

click the arrow and select Auto.<br />

The options available in the IVIS acquisition control panel depend on the selected<br />

imaging mode, the imaging system, and the filter wheel or lens option that are<br />

installed.<br />

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