Living Image 3.1

Index
luciferase spectrum 227
optical properties 227
results 110
tools 109–110
point source fitting 129–133
results 134–135
tools 129–132
preferences 181–189
pseudocolor image 197
R
radiance
photon 200
radiance units 200
read bias 203
reconstruct 3D fluorescent sources 142–143
reconstruct 3D luminescent sources 138–141
reduced Chi2 145
ROI 69
automatically draw 76–77
background-corrected signal 79–81
delete 90
edit dimensions 86
edit position 86
free draw 78
managing 82
manually draw 75–76
measurement ROI
free draw 78
measurement ROIs 74–77
Measurements table 74
move 85
move or edit label 88
quick guide 73
ROI line 87
save 89
subject 78
tools 71–72
ROI Measurements table 91–93
configure 93–95
copy or export 95
ROI properties 82–84
ROI types
average background 70, 79
measurement 70
subject 70
250
S
save
ROI 89
save data manually 33
scientific image data 197
segment 28
smoothing 55, 56, 194
source spectrum 146
spectral imaging
See planar spectral imaging.
spectral unmixing 113–116
options 120–121
parameters 118–119
PCA biplot 122
PCA explained variance 123
results 117–118
starting the software 9
starting the system
See system initialization.
steradian 200
subject ROI 70, 78
surface topography 125–126
system
FOV 180
initialization 11
optics autofluorescence 221
temperature 12
T
tag an image 48
technical support 3
temperature 12
threshold angle 145
tile images 48
tissue autofluorescence 223–224
eliminate by spectral unmixing 113–116
subtracting with background filters 101–103
tissue properties 146
tool palette 40
3D tools 148–173
correcting/filtering 55–56
image information 56–57
point source fitting 129–132
ROI tools 71
toolbar 243
total source flux 144
transillumination overview 65