Living Image 3.1

Living Image ® Software User’s Manual
2
Getting Started
2.1 Overview of Imaging & Image Analysis
Overview of Imaging & Image Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Starting the Living Image ® Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Living Image Help . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
This chapter provides a brief overview of images and image analysis. It also explains
how to start the Living Image ® software.
For bioluminescence imaging, the imaging system acquires a photographic image and a
bioluminescence image. The Living Image software automatically coregisters the
images to generate an overlay image (Figure 2.1). For fluorescence imaging, the IVIS ®
imaging system acquires a photographic and fluorescence image that are used to
generate an overlay image.
Table 2.1 Images used to generate an overlay image
Image Type Description
Photographic A short exposure of the subject illuminated by the lights located at the
top of the imaging chamber (Figure 2.2). The photographic image is
displayed as a grayscale image.
Bioluminescence A longer exposure of the subject taken in darkness to capture low level
bioluminescence emission. The bioluminescence image is displayed in
pseudocolor that represents intensity (Figure 2.1). For more details on
bioluminescence image data, see Appendix D, page 197.
Fluorescence An exposure of the subject illuminated by filtered light. The target
fluorophore emission is captured and focused on the CCD camera.
Fluorescence image data can be displayed in units of counts or photons
(absolute, calibrated), or in terms of efficiency (calibrated, normalized).
For more details on fluorescence image data, see Appendix F,
page 209
Figure 2.3 shows an example workflow on an IVIS ® Imaging System.
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