Living Image 3.1

Living Image ® Software User’s Manual
4.11 Viewing Transillumination Data
The transillumination overview feature combines the images of a FLIT sequence (a
fluorescence sequence acquired in transillumination mode) into a single image. All of
the individual fluor signals are stacked over one photograph and the intensity is
summed. One overview is created per filter pair. If two filter pairs were used during
acquisition, then two overview images will be created. All transillumination locations
are displayed simultaneously; a tool tip displays the transillumination position when
you mouse over a transillumination point.
An overview image is displayed in photon units and can be analyzed using the tools in
the tool palette.
1. Open a sequence acquired in fluorescence transillumination mode.
2. Select Tools → Transillumination Overview for xx_SEQ on the menu bar.
- The overview appears.
Sequence view Transillumination overview
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