Living Image 3.1

Living Image ® Software User’s Manual
1. In the tool palette, click DLIT 3D Reconstruction.
2. In the Properties tab, make a selection from the
“Tissue Properties” and “Source Spectrum”
drop-down lists.
“Muscle” is usually the best choice of tissue
properties for general in vivo reconstructions.
Note: The internal medium index of refraction is
automatically entered when you select a tissue.
3. If you want to view the “Tissue Properties” (μa, μeff , μ ’ s ) or “Source Spectrum” for the tissue and
light source selected above, make a selection
from the Plot drop-down list in the Properties
tab.
4. In the Analyze tab, select the acquisition
wavelengths (560-660 nm).
5. If necessary, edit the minimum radiance
associated with an acquisition wavelength or
angle. For more details on the minimum
radiance, see page 239.
Note: It is recommended that you only analyze
images that have signal well above the noise.
6. To edit the minimum radiance, double-click the
entry and enter a new value.
Note: The minimum radiance level can be
observed by looking at individual images and
adjusting the min level on the color bar, in
radiance units (photons/sec/cm 2).
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