Living Image 3.1

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E. Luminescent Background Sources & Corrections 208 Photograph Luminesce nt overlay Photograph Luminescent overlay Figure E.2 Background light emission Background light emission from a female white furred (Swiss Webster) (left) and a female nude (Nu/nu) mouse (right). Usually only very low signals at the highest level of sensitivity require this type of background subtraction. For more information on how best to handle these types of measurements, please contact Xenogen technical support.
Living Image ® Software User’s Manual Appendix F Fluorescent Imaging F.1 Description and Theory of Operation Description and Theory of Operation . . . . . . . . . . . . . . . . . . . . . . 209 Filter Spectra . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 211 Working with Fluorescent Samples . . . . . . . . . . . . . . . . . . . . . . . 213 Image Data Display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 214 Fluorescent Background . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 215 Subtracting Instrument Fluorescent Background . . . . . . . . . . . . . . . . 221 Adaptive Background Subtraction . . . . . . . . . . . . . . . . . . . . . . . . 221 Subtracting Tissue Autofluorescence Using Background Filters . . . . . . . . 223 System Components The IVIS ® Imaging System 200 Series and IVIS Lumina offer built-in fluorescence imaging capability as standard equipment. The IVIS Imaging System 100 and 50 Series use the XFO-6 or XFO-12 Fluorescence Option to perform fluorescence imaging. The fluorescence equipment enables you to conveniently change between bioluminescent and fluorescent imaging applications (Figure F.1). For more details, see the IVIS Imaging System 200 Series System Manual, the IVIS Lumina System Manual, or the XFO-6 or XFO- 12 Fluorescence Option Manual. IVIS Imaging System 200 Series Figure F.1 Fluorescent imaging hardware IVIS Lumina, IVIS Imaging System 50 or 100 Series A 150-watt quartz tungsten halogen (QTH) lamp with a dichroic reflector provides light for fluorescence excitation. The relative spectral radiance output of the lamp/reflector 209
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Living Image 3.1

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