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Living Image 3.1

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<strong>Living</strong> <strong>Image</strong> ® Software User’s Manual<br />

3. If this is the first acquisition of the<br />

session, you are prompted to<br />

choose an autosave location.<br />

4. To specify a folder for autosaved data, click Yes in the prompt and choose a folder<br />

in the dialog box that appears.<br />

- All sequences acquired during the session are automatically saved to this folder.<br />

You can choose a different folder at any time (select Acquisition → Auto-Save<br />

on the menu bar).<br />

5. In the Edit <strong>Image</strong> Labels box that appears, enter information for the image label and<br />

click OK (Figure 3.3). If you do not want to enter label information, click Cancel.<br />

Figure 3.4 <strong>Image</strong> window & Edit <strong>Image</strong> Label box<br />

<strong>Image</strong> label<br />

Open an image (double-click the thumbnai<br />

determine whether the signal of interest i<br />

above the noise level and below CCD<br />

saturation.<br />

Check the image min and max in the color<br />

A signal greater than 600 counts and less t<br />

~60,000 counts is recommended. If the sig<br />

level is unacceptable, adjust the exposure<br />

time or binning level.<br />

For more details on the image window, see<br />

Table 3.7<br />

Edit the label information here.<br />

31

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