Living Image 3.1

Living Image ® Software User’s Manual
7
Planar Spectral Image Analysis
7.1 Planar Spectral Image Analysis
Planar Spectral Imaging Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109
Planar Spectral Image Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
Viewing & Exporting Graphical Results . . . . . . . . . . . . . . . . . . . . . . . . . . . 111
Managing Planar Spectral Imaging Results . . . . . . . . . . . . . . . . . . . . . . . . . 112
The Living Image ® software enables you to apply planar spectral image analysis to a
sequence to determine the average depth and total photon flux of a bioluminescent point
source in a user-specified region of interest. For more information on planar spectral
image analysis, see Appendix G, page 225.
Use the imaging wizard to setup the image sequence required for planar spectral image
analysis. (For more details on the imaging wizard, see page 23.) At a minimum, the
sequence must include a photographic and luminescent image at the first wavelength
and a luminescent image at a second wavelength (560-660).
1. Open the image sequence that you want to analyze.
2. In the tool palette, click Planar Spectral Imaging.
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