Living Image 3.1

Living Image ® Software User’s Manual
Appendix F Fluorescent Imaging
F.1 Description and Theory of Operation
Description and Theory of Operation . . . . . . . . . . . . . . . . . . . . . . 209
Filter Spectra . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 211
Working with Fluorescent Samples . . . . . . . . . . . . . . . . . . . . . . . 213
Image Data Display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 214
Fluorescent Background . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 215
Subtracting Instrument Fluorescent Background . . . . . . . . . . . . . . . . 221
Adaptive Background Subtraction . . . . . . . . . . . . . . . . . . . . . . . . 221
Subtracting Tissue Autofluorescence Using Background Filters . . . . . . . . 223
System Components The IVIS ® Imaging System 200 Series and IVIS Lumina offer built-in fluorescence
imaging capability as standard equipment. The IVIS Imaging System 100 and 50 Series
use the XFO-6 or XFO-12 Fluorescence Option to perform fluorescence imaging. The
fluorescence equipment enables you to conveniently change between bioluminescent and
fluorescent imaging applications (Figure F.1). For more details, see the IVIS Imaging
System 200 Series System Manual, the IVIS Lumina System Manual, or the XFO-6 or XFO-
12 Fluorescence Option Manual.
IVIS Imaging System 200 Series
Figure F.1 Fluorescent imaging hardware
IVIS Lumina,
IVIS Imaging System 50 or 100 Series
A 150-watt quartz tungsten halogen (QTH) lamp with a dichroic reflector provides light
for fluorescence excitation. The relative spectral radiance output of the lamp/reflector
209