Industrial Biotransformations

108
4 Optimization of Industrial Enzymes by Molecular Engineering
4.6
Conclusions and Future Prospects
In this chapter strategies and methods for molecular enzyme engineering have been presented
and their pros and cons have been discussed. For further information three
recently published books are recommended [84, 88, 89] as they give detailed insights into
(random-) mutagenesis and high-throughput screening methods.
Although directed evolution works even without any knowledge of an enzymes’ structure
or reaction mechanism, knowledge of the three-dimensional protein structure can
significantly speed up a directed evolution approach because the size of the sequence
space to be sampled can be narrowed down. Eventually, a structure- and theory-assisted
method of molecular enzyme engineering using directed evolution will facilitate the creation
of better performing biocatalysts for industrial applications.
Acknowledgments
The author thanks Prof. Karl-Erich Jaeger and P.D. Martina Pohl (Institute for Molecular
Enzyme Technology, University of Düsseldorf, Germany) for critical reading of the manuscript
and valuable advice.
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