Industrial Biotransformations

Glutaryl amidase
Pseudomonas sp.
3) Flow scheme
pH
Fig. 3.1.1.41 – 2
4) Process parameters
yield: 95 %
reactor type: repetitive batch, fixed bed reactor
reactor volume: 1,000 L
capacity: 90 t · a –1
residence time: 4 h
start-up date: 1973
company: Asahi Kasei Chemical Industry Co., Ltd., Japan, and Toyo Jozo, Japan
5) Product application
● 7-ACA is an intermediate for semisynthetic cephalosporins.
6) Literature
NaOH H
HO
N
HO
O
E
O
OH
EC 3.1.1.41
● Christ, C. (1995) Biochemical production of 7-aminocephalosporanic acid, in: Ullmann’s Encyclopedia
of Industrial Chemistry, Vol. B8 (Arpe, H.-J. ed.) pp. 240–241, VCH Verlagsgesellschaft,
Weinheim
● Matsumoto, K. (1993) Production of 6-APA, 7-ACA, and 7-ADCA by immobilized penicillin
and cephalosporin amidases, in: Industrial Application of Immobilized Biocatalysts (Tanaka,
A, Tosa, T., Kobayashi, T. eds.) pp. 67–88, Marcel Dekker Inc., New York
● Tsuzuki, K., Komatsu, K., Ichikawa, S., Shibuya, Y. (1989) Enzymatic synthesis of 7-aminocephalosporanic
acid (7-ACA), Nippon Nogei Kagaku Kaishi 63 (12), 1847
● Verweij, J., de Vroom, E. (1993) Industrial transformations of penicillins and cephalosporins,
Rec. Trav. Chim. Pays-Bas 112 (2), 66–81
H 2N
O
O
N
S
O
COOH
O
O
O
N
S
COOH
O
O
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