Industrial Biotransformations

Nucleosidase / Phosphorylase
Erwinia carotovora
HOCH2
1) Reaction conditions
T: 60 °C
medium: aqueous
reaction type: N-glycosyl bond hydrolysis / pentosyl group transfer
catalyst: solubilized enzyme
enzyme: purine nucleosidase / purine – nucleoside phosphorylase
strain: Erwinia carotovora
CAS (enzyme): [9025–44–9] / [9055–35-0]
2) Remarks
● The enzymes were isolated and purified from Erwinia carotovora.
● Alternatively biocatalysts from Brevibacterium acetylicum and Bacillus megaterium can be used.
3) Flow scheme
Not published.
O
H
N O
O
N
HO OH
H2N
R
1 2
O
4
HOCH2
O
HO OH
OPO 3-
HOCH 2
EC 3.2.2.1/2.4.2.2
1 = R = H: uridine, R = COOH: orotidine
2 = R1 = NH2, R2 = H: adenosine; R1 = O, R2 = NH2,: guanosine
3 = ribose-1-phosphate
4 = 1,2,4-triazole-3-carboxamide
5 = ribavirin Yamasa Corporation
Fig. 3.2.2.1/2.4.2.2 – 1
N N N
E1
Purine nucleosidase
- O
O -
P
O
3
O -
E1
Purine nucleosidase
Pyrimidine nucleoside phosphorylase
E2
- O
R 2
O
N
N
HO OH
N
HOCH2
O
N
O -
P
O
O -
HO OH
5
N
CONH2
N
R 1
N
335