Industrial Biotransformations

GlcNAc 2-epimerase
Escherichia coli
4) Process parameters
conversion: 84 %
reactor type: batch
reactor volume: 200 L
capacity: multi kg
down stream processing: subsequent, in situ biotransformation to N-acetylneuraminic acid
enzyme supplier: Marukin Shoyu Co., Ltd., Japan
company: Marukin Shoyu Co., Ltd., Japan
5) Product application
● N-Acetyl-d-mannosamine serves as in situ generated substrate for the synthesis of N-acetylneuraminic
acid.
6) Literature
EC 5.1.3.8
● Kragl, U., Gygax, D., Ghisalba, O., Wandrey, C. (1991) Enzymatic process for preparing N-acetylneuraminic
acid, Angew. Chem. Int. Ed. Engl. 30, 827–828
● Maru, I., Ohnishi, J., Ohta, Y., Tsukada, Y. (1998) Simple and large-scale production of N-acetylneuraminic
acid from N-acetyl-d-glucosamine and pyruvate using N-acyl-d-glucosamine 2epimerase
and N-acetylneuraminate lyase, Carbohydrate Res. 306, 575–578
● Maru, I., Ohta, Y., Murata, K., Tsukada, Y. (1996) Molecular cloning and identification of Nacyl-d-glucosamine
2-epimerase from porcine kidney as a renin-binding protein, Biol. Chem.
271, 16294–16299
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