Industrial Biotransformations

HOOC
HO
HOOC
O
NH 2
NH 2
O
chemical
O
H
N
H
N
O
glutaryl amidase
H 2N
O
O
1.2 From Gastric Juice to SweetzymeT – The History of Enzymatic Biotransformations
N
N
cephalosporin C
glutaryl-7-ACA
O
7-ACA
H
N
O
N
H 2 N
O
+ H 2O 2
– CO 2
– NH 3
N
S
S
S
S
– HO
COOH
COOH
E
N
2
COOH
COOH
S
O
O
COOH
O
O
O
O
HO
O
O
O
O
O
OH
E
O
H
N
O
Fig. 1.27 Two-step process for production of 7-ACA
from cephalosporin C.
O 2
N
S
COOH
Fig. 1.28 Flow-scheme for the production of 7-ACA. Production
carried out at Asahi Chemical Industry (E 1 = d-amino acid oxidase;
E 2 = glutaryl amidase).
O
O
1
O
O
21