Industrial Biotransformations

Aminopeptidase
Pseudomonas putida
EC 3.4.11.1
● Using in vivo protein engineering not only mutant strains of Pseudomonas putida exhibiting Lamidase
and also D-amidase but also amino acid amide racemase activities were obtained.
Using these mutants a convenient synthesis of α-H-amino acids with 100 % yield would be
possible with a single cell system.
● It is noteworthy that only α-H-substrates can be used. By screening techniques a new biocatalyst
of the strain Mycobacterium neoaurum was found, which is capable of converting α-substituted
amino acid amides. The next figure shows possible substrates for Mycobacterium
neoaurum:
R 1 = CH3
R 2 =
Fig. 3.4.11.1 – 5
340
O
O
O
R 1
R 2
O
NH2
NH2
R 2 =
R 1 =