Industrial Biotransformations

Lactonase
Fusarium oxysporum
1 = pantolactone
2 = pantoic acid
Fig. 3.1.1.25 – 1
1) Reaction conditions
[1]: 2.69 M, 350 g · L –1 [130.14 g · mol –1 ]
pH: 6.8 – 7.2
T: 30 °C
medium: aqueous
reaction type: carboxylic ester hydrolysis
catalyst: immobilized whole cells
enzyme: 1,4-lactone hydroxyacylhydrolase (γ-lactonase)
strain: Fusarium oxysporum
CAS (enzyme): [37278–38–9]
2) Remarks
2
O
OH
O
E
+H2O
D,L-1 D-2
racemization
OH
HO
● The reverse reaction, the lactonization of aldonic acid, is catalyzed under acidic conditions.
The reverse reaction does not take place with aromatic substrates.
● The lactonase from Fusarium oxysporum has a very broad substrate spectrum:
OH
O
+
O
L-1
OH
EC 3.1.1.25
Fuji Chemical Industries Co., Ltd.
O
321