Industrial Biotransformations

Aldehyde reductase
Escherichia coli
1 = ethyl-4,4,4-trifluoracetoacetate
2 = (R)-ethyl-4,4,4-trifluoro-3-hydroxybutanoate
Fig. 1.1.1.2 – 1
1) Reaction conditions
[1]: [184.11 g · mol –1 ]
medium: two-phase system: water / butyl acetate
reaction type: redox reaction
catalyst: whole cells
enzyme: aldehyde reductase
enzyme: glucose dehydrogenase
strain: Escherichia coli JM109
2) Remarks
● The production strain contains two plasmids. One carries the aldehyde reductase gene from
Sporobolomyces salmonicolor, which catalyzes the reduction step. The second one contains a glucose
dehydrogenase gene from Bacillus megaterium, which enables cofactor regeneration of
NADPH from NADP.
● The production strain is grown at 22 °C to avoid formation of inclusion bodies. Cells are harvested,
washed and stored frozen until use.
● The process is carried out in a water / butyl acetate two-phase system to avoid inhibition of the
reductase by the substrate and product.
3) Flow scheme
Not published.
1 2
4) Process parameters
yield: 50 %
ee: 99 %
company: Lonza, Switzerland
174
F 3C
O
CO 2Et
E
NADPH + H + NADP +
F 3C
OH
CO 2Et
EC 1.1.1.2
Lonza