Industrial Biotransformations

Carnitine dehydratase
Escherichia coli
H2O
COO- N + COO- N +
N +
Acetyl-CoA
1 2
ATP + HSCoA
N +
O
7
8
AMP + PPi
O
O
E 7
O -
citric acid cycle
SCoA
E 1
1 = 4-butyrobetaine
2 = crotonobetaine
3 = 4-butyrobetainyl-CoA
4 = crotonobetainyl-CoA
5 = carnityl-CoA
6 = carnitine
7 = dehydrocarnityl-CoA
8 = betaine
E1= 4-butyrobetainyl-CoA-synthetase
E 2= 4-butyrobetainyl-CoA-dehydrogenase
E 3= crotonobetainyl-CoA-synthetase
E 4= crotonobetainyl-CoA-hydrolase
E 5= thioesterase
E 6= carnitine-dehydrogenase
E 7= carnitine hydrolase
Fig. 4.2.1.89 – 2
N +
3
FAD
FADH2
O
SCoA
E 6
E 2
N +
N +
N +
E 3
E4
E 5
4
OH
L-5
OH
L-6
O
O
O
EC 4.2.1.89
ATP
AMP + PPi
SCoA
H 2O
SCoA
H 2O
HSCoA
O -
489