Industrial Biotransformations

Aminoacylase
Aspergillus oryzae
3) Flow scheme
Fig. 3.5.1.14 – 3
4) Process parameters
yield: 80 %
ee: 99.5 %
reactor type: cstr, UF-membrane reactor
capacity: > 300 t · a –1 Drauz (Artikel-Korrektur)
residence time: 2.9 h
space-time-yield: 592 g · L –1 ·d –1
down stream processing: crystallization
enzyme activity: 1,067 U · g –1 enzyme consumption:
protein
2,067 U · kg –1
enzyme supplier: Amano Enzymes, Inc., Japan
production site: Degussa-Rexime, China
company: Degussa AG, Germany
5) Product application
● l-Amino acids are used for parenteral nutrition (infusion solutions), feed and food additives,
cosmetics, pesticides and as intermediates for pharmaceuticals as well as chiral synthons for
organic synthesis.
6) Literature
EC 3.5.1.14
● Bommarius, A.S., Drauz, K., Klenk, H., Wandrey, C. (1992) Operational stability of enzymes –
acylase-catalyzed resolution of N-acetyl amino acids to enantiomerically pure l-amino acids,
Ann. N. Y. Acad. Sci. 672, 126–136
● Chenault, H.K., Dahmer, J., Whitesides, G.M. (1989) Kinetic resolution of unnatural and rarely
occuring amino acids: enantioselective hydrolysis of N-acyl amino acids catalyzed by acylase I,
J. Am. Chem. Soc. 111, 6354–6364
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