Industrial Biotransformations

Fumarase
Brevibacterium flavum
4) Process parameters
conversion: 80 % (equilibrium conversion)
yield: > 70 %
reactor type: plug-flow reactor
reactor volume: 1,000 L
capacity: 468 t · a –1
enzyme activity: 17 U · mL(gel) –1 (37 °C); 28 U · mL(gel) –1 (50 °C)
enzyme consumption:
start-up date:
t1/2 = 243 d (37 °C); t1/2 = 128 d (50 °C)
1974
company: Tanabe Seiyaku Co., Ltd., Japan
5) Product application
● The product is used as an acidulant in fruit and vegetable juices, carbonated soft drinks, jams
and candies, in amino acid infusions and for the treatment of hepatic malfunctioning.
6) Literature
● Tosa, T., Shibatani, T. (1995) Industrial applications of immobilized biocatalysts in Japan, Ann.
N. Y. Acad. Sci. 750, 364–375
● Tanaka, A., Tosa, T., Kobayashi, T. (1993) Industrial Application of Immobilized Biocatalysts,
Marcel Dekker Inc., New York
● Lilly, M.D. (1994) Advances in biotransformation processes. Eighth P. V. Danckwerts memorial
lecture presented at Glaziers’ Hall, London, U.K. 13 May 1993, Chem. Eng. Sci. 49, 151–159
● Wiseman, A. (1995) Handbook of Enzyme and Biotechnology, Ellis Horwood, Chichester
● Sheldon, R.A. (1993) Chirotechnology, Marcel Dekker Inc., New York
EC 4.2.1.2
● Crosby, J. (1991) Synthesis of optically active compounds: a large scale perspective, Tetrahedron
47, 4789–4846
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