Industrial Biotransformations

Benzoate dioxygenase
Pseudomonas putida
● The cofactor regeneration of NADH takes place cell intracellularly, driven by the addition of a
carbon source.
3) Flow scheme
Not published.
4) Process parameters
selectivity: > 99.5 %
capacity: several t · a –1
company: ICI, U.K., and others
5) Product application
● cis-Dihydrodiols are used as chiral intermediates in the synthesis of β-lactams that are effective
as antiviral compounds.
● The product is also used as a polymerisation monomer.
6) Literature
● Crosby, J. (1991) Synthesis of optically active compounds: A large scale perspective, Tetrahedron
47, 4789–4846
● Evans, C., Ribbons, D., Thomas, S., Roberts, S. (1990) Cyclohexenediols and their use, Enzymatix
Ltd., WO 9012798 A2
● Sheldrake, G.N. (1992) Biologically derived arene cis-dihydrodiols as synthetic building blocks,
in: Chirality in Industry (Collins, A.N., Sheldrake, G.N., Crosby, J., eds.) pp. 127–166, John
Wiley & Sons, New York
● Taylor, S.C. (1983) Biochemical Process, ICI, EP 76606 B1
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EC 1.14.12.10