Industrial Biotransformations

Cyclohexanone monooxygenase
Acinetobacter calcoaceticus
3) Flow scheme
4) Process parameters
conversion: 92 %
yield: 76 %
optical purity 98 %
reactor type: CSTR
reactor volume: 55 L
capacity: kg-scale
space-time-yield: 1.1 g · L –1 ·h –1
down stream processing: centrifugation, adsorption on active charcoal, chromatography on silica
gel, crystallization
enzyme activity: 5 gcdw ·L –1 / 400 U · g –1 / -> 2000 U · L –1
enzyme supplier: Fluka, Buchs, Switzerland
production site: Fluka AG, Switzerland
company: Sigma Aldrich, USA
5) Product application
● The applied reaction is used as a model reaction. The feasibility of using microbiology as an
alternative for Baeyer–Villiger oxidations was demonstrated.
6) Literature
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Fig. 1.14.13.22 – 2
EC 1.14.13.22
● Doig, S.D., Avenell, P.J., Bird, P.A., Gallati, P., Lander, K.S., Lye, G.J., Wohlgemut, R., Woodley,
J.M. (2002) Reactor operation and scale-up of whole cell Baeyer-Villiger catalyzed lactone
synthesis, Biotechnol. Proc. 18, 1039–1046
● Alphand, V., Carrea, G., Wohlgemut, R., Furstoss, R., Woodley, J.M. (2003) Towards large-scale
synthetic applications of Baeyer-Villiger monooxygenases, Trends Biotechnol. 21, (7), 318–323
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chromatography
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