Industrial Biotransformations

Lactonase
Fusarium oxysporum
O
OH
HCOH
OH
CH2OH O
O
D,L-galoctono-γ-lactone
O
HO OH
HOCH
CH2OH D,L-mannono-γ-lactone
O
OH OH
HOCH
CH2OH
O
HCOH COH
OH
H
OH
CH2OH D,L-glycero-D-gulo-heptono-γ-lactone
O
HOCH OH
HCOH
CH2OH
OH
O
D,L-gulono-γ-lactone
D,L-glycero-L-manno-heptono-γ-lactone
Fig. 3.1.1.25 – 2
O
O
HOCH
HOCH
CH 2OH
HCOH
O
OH
CH2OH
O
EC 3.1.1.25
● For the synthesis whole cells are immobilized in calcium alginate beads and used in a fixed
bed reactor.
● The immobilized cells retain more than 90 % of their initial activity even after 180 days of continuous
use.
● At the end of the reaction l-pantolactone is extracted and reracemized to d,l-pantolactone that
is recycled into the reactor. The d-pantoic acid is chemically lactonized to d-pantolactone and
extracted:
322
OH
O
O
α,β-glucooctanoic-γ-lactone
OH OH
D,L-ribono-γ-lactone
OH
OH
O
OH
CH2OH
O
OH
O
OH
OH
O
D,L-erythrono-γ-lactone
O
D,L-glucono-δ-lactone
O
D,L-pantolactone
HO
O
O
O
O
O
dihydrocoumarin
homogentistic acid
lactone
O
2-coumaranone
O
3-isochromanone
O