Industrial Biotransformations

Alcohol dehydrogenase
Rhodococcus erythropolis
● Using this method very good space-time yields are obtainable in spite of the low substrate solubilities:
conversion (%)
space-time yield (g*L -1 *d -1 )
consumption ADH (U*kg -1 )
consumption FDH (U*kg -1 )
ttn
Fig. 1.1.1.1 – 3
3) Flow scheme
Fig. 1.1.1.1 – 4
OH
4) Process parameters
conversion: 72 %
yield: 72 %
selectivity: 100 %
ee: >99 %
reactor type: CSTR (enzyme bimembrane reactor)
reactor volume: 0.05 L
residence time: 0.33 h
space-time-yield: 63.5 g . L –1. d –1
down stream processing: distillation
enzyme activity: 0.95 U . mL –1
enzyme consumption: 3.5 U . g –1
enzyme supplier: Institute of Molecular Enzyme Technology, Germany
start-up date: 1996
production site: Forschungszentrum Jülich GmbH, Germany
company: Forschungszentrum Jülich GmbH, Germany
158
O
EC 1.1.1.1
(S)-1-phenyl-propan-2-ol (S)-4-phenyl-butan-2-ol (S)-6-methyl-hept-5-en-2-ol
E
72 80 65
64
3,540
10,200
1,350
enzyme
l
104
3,025
4,860
UFmembrane
158
ll
OH
water
hexane
hydrophobic
membrane
lll
60
not det.
not det.
747
OH
OH