Industrial Biotransformations

Aspartate b-decarboxylase
Pseudomonas dacunhae
4) Process parameters
conversion: 99 %
yield: 86 % after down stream processing
reactor type: plug flow reactor
reactor volume: 1,000 L (each fixed bed column)
capacity: 114 t · a –1 d-aspartic acid; 61 t · a –1 l-alanine
residence time: 11 h
space-time-yield: 317 g · L –1 ·d –1 d-aspartic acid; 170 g · L –1 ·d –1 l-alanine
down stream processing: crystallization
start-up date: 1982
production site: Tanabe Seiyaku Co., Ltd., Japan
company: Tanabe Seiyaku Co., Ltd., Japan
5) Product application
● The products are used in infusion solutions and as food additives.
● d-Aspartic acid is an intermediate for the semi-synthetic penicillin apoxycillin:
Fig. 4.1.1.12 – 6
6) Literature
EC 4.1.1.12
● Chibata, L., Tosa, T., Shibatani, T. (1992) The industrial production of optically active compounds
by immobilized biocatalysts, in: Chirality in Industry (Collins, A.N., Sheldrake, G.N.,
Crosby, J., eds.) pp. 351–370, John Wiley & Sons, New York
● Furui, M., Yamashita, K. (1983) Pressurized reaction method for continuous production of lalanine
by immobilized Pseudomonas dacunhae cells, J. Ferment. Technol. 61, 587–591
● Schmidt-Kastner, G., Egerer, P. (1984) Amino acids and peptides, in: Biotechnology, Vol. 6a,
(Kieslich, K., ed.) pp. 387–419, Verlag Chemie, Weinheim
O
● Takamatsu, S., Umemura, I., Yamamoto, K., Sato, T., Tosa, T., Chibata, I. (1982) Production of
l-alanine from ammonium fumarate using two immobilized microorganisms: elimination of
side reactions, Eur. J. Appl. Microbiol. Biotechnol. 15, 147–152
● Takamatsu, S., Tosa, T. (1993) Production of l-alanine, in: Industrial Application of Immobilized
Biocatalysts, (Tanaka, A., Tosa, T., Kobayashi, T., eds.) Marcel Dekker Inc., New York
454
H
N
O
O
D
*
NH 2
N
H
OH
D *
O
apoxycillin
H
N
N
S
COOH