Industrial Biotransformations

Monooxygenase
Nocardia corallina
2) Remarks
● The alkene monooxygenase catalyzes the epoxidation of terminal and sub-terminal alkenes:
Fig. 1.14.14.1 – 2
H
H
O
O
O
n (n >_ 0)
n (n >_ 0)
n
(n = 4, 5...)
● The stereospecific epoxidation yields predominantly the (R)-enantiomer.
● The epoxidation activity is formed constitutively since the conversion takes place independent
of the type of carbon source used (alkene or glucose).
● The biotransformation is carried out in a conventional fermentation system.
O
O
n (n >_ 4)
(n = 1, 2...)
EC 1.14.14.1
● The substrates have to be divided in three main classes: short-chain gaseous (C 3-C 5), short
chain – liquid (C 6-C 12), long-chain (C >12) olefins.
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Cl F
F
F
Me OMe
Me
O
Cl
O O
n
F
F
F
F
O
F