Industrial Biotransformations

Trypsin
Pig Pancreas
2) Remarks
● The precursor pro-insulin is directly produced by fermentation of recombinant E. coli (see second
step of process on page 344).
● See page 346 for an overview of possible syntheses of insulin.
3) Flow scheme
● See page 243.
4) Process parameters
yield: > 70 %
reactor type: batch
company: Eli Lilly, USA
5) Product application
● See process of Hoechst Martion Roussel on page 346.
6) Literature
EC 3.4.21.4
● Frank, B.H., Chance, R.E. (1983) Two routes for producing human insulin utilizing recombinant
DNA technology, Münch. Med. Wschr. 125, 14–20
● Jørgensen, L.N., Rasmussen, E., Thomsen, B. (1989) HM(ge), Novo’s biosynthetic insulin;
Med. View. III, No. 4, 1–7
● Ladisch, M.R., Kohlmann, K.L. (1992) Recombinant human insulin, Biotechnol. Prog. 8, 469–
478
351