Industrial Biotransformations

Nitrilase / Hydroxylase
Alcaligenes faecalis
3) Flow scheme
Not published.
4) Process parameters
conversion: 100 %
yield: 87 %
reactor type: fed-batch
capacity: 1 t · a –1
residence time: 31 h
space-time-yield: 58 g · L –1 ·d –1
down stream processing: precipitation
company: Lonza AG, Switzerland
5) Product application
● The product is used as an intermediate for pharmaceuticals, e.g. 2-oxypyrimidine, and herbicides.
6) Literature
EC 3.5.5.1 / 1.5.1.13
● Fischer, E., Heß, K., Stahlschmidt, A. (1912) Verwandlung der Dihydrofurandicarbonsäure in
Oxypyridincarbonsäure, Ber. Dtsch. Chem. Ges. 45, 2456–2467
● Foster, C.J., Gilkerson, T., Stocker, R. (1991) Herbicidal carboxamide derivatives, Shell International
Research Maatschappij B.V., EP 0447004 A2
● Glöckler, R., Roduit, J.-P. (1996) Industrial bioprocesses for the production of substituted aromatic
heterocycles, Chimia 50, 413–415
● Kiener, A. (1992) Mikrobiologisches Verfahren zur Herstellung von 6-Hydroxypicolinsäure,
Lonza AG, EP 0504818 A2
● Kiener, A., Glöckler, R., Heinzmann, K. (1993) Preparation of 6-O-oxo-1,6-dihydropyridine-2carboxylic
acid by microbial hydroxylation of pyridine-2-carboxylic acid, J. Chem. Soc. Perkin
Trans. I 11, 1201–1202
● Kiener, A., Roduit, J.-P., Glöckler, R. (1999) Mikrobiologisches Verfahren zur Herstellung von
heteroaromatischen Carbonsäuren mittels Mikroorganismen der Gattung Alcaligenes, Lonza
AG, EP 0747486 B1
● Petersen, M., Kiener, A. (1999) Biocatalysis – preparation and functionalization of N-heterocycles,
Green Chem. 2, 99–106
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