Industrial Biotransformations

Amidase
Klebsiella oxytoca
company: Lonza AG, Switzerland
5) Product application
● The (R)- and (S)-acids, respectively, are intermediates for the synthesis of a number of potential
pharmaceuticals, which include ATP-sensitive potassium channel openers for the treatment of
incontinence and inhibitors of pyruvate dehydrogenase kinase for treatment of diabetes.
6) Literature
EC 3.5.1.4
● Shaw, N.M., Naughton, A., Robins, K., Tinschert, A., Schmid, E., Hischier, M.-L., Venetz, V.,
Werlen, J., Zimmermann, T., Brieden, W., de Riedmatten, P., Roduit, J.-P., Zimmermann, B.,
Neumüller, R. (2002) Selection, purification, characterization, and cloning of a novel heatstable
stereo-specific amidase from Klebsiella oxytoca, and its application in the synthesis of
enantiomerically pure (R)- and (S)-3,3,3-trifluoro-2-hydroxy-2-methylpropionic acids and (S)-
3,3,3-trifluoro-2-hydroxy-2-methylpropionamide, Org. Proc. Res. Dev. 6, 497–504
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