Industrial Biotransformations

Penicillin amidase
Bacillus megaterium
1) Reaction conditions
[1]: 0.3 M, 100 g · L –1 [334.39 g · mol –1 ]
pH: 8.4
T: 30–36 °C
medium: aqueous
reaction type: carboxylic acid amide hydrolysis
catalyst: immobilized enzyme
enzyme: penicillin amidohydrolase (penicillin acylase, penicillin amidase)
strain: Bacillus megaterium
CAS (enzyme): [9014-06–6]
2) Remarks
H 2O
1 2
● The enzyme from Bacillus megaterium is an exoenzyme and immobilized using an aminated
porous polyacrylonitrile (PAN) fiber as solid support.
● The production is carried out in a recirculation reactor consisting of 18 parallel columns with
immobilized enzyme. Each column has a volume of 30 L. The circulation of the reaction solution
is established with a flow rate off 6,000 L · h –1 . One cycle time takes 3 hours.
● The lifetime of each column is 360 cycles.
EC 3.5.1.11
● Purification of 6-APA is done by isoelectric precipitation at pH 4.2 with subsequent filtration
and washing with methanol.
H 2N
O
N
S
COOH
1 = penicillin-G
2 = 6-amino penicillanic acid ( 6-APA)
3 = phenylacetic acid Asahi Kasei Chemicals Corporation
Fig. 3.5.1.11 – 1
H
N
O N
O
S
COOH
E
+
3
COOH
391