Industrial Biotransformations

Nitrilase
Escherichia coli
1) Reaction conditions
[1]: [133.15 g · mol –1 ]
pH: 7.2
T: 40 °C
medium: aqueous
enzyme: nitrile aminohydrolase (nitrilase)
strain: E. coli JM (pDHE19.2)
CAS (enzyme): [9024-90-2]
2) Flow scheme
Not published.
3) Process parameters
ee: >99 %
reactor type: batch
capacity: multi-ton scale
company: BASF AG, Germany
4) Literature
1 2
EC 3.5.5.1
1 = (R,S)-cyanohydrine
2 = (R)-mandelic acid BASF AG
Fig. 3.5.5.1 – 1
OH OH
E
CN CO 2H
+ 2 H 2O
- NH 3
● Gröger, H. (2001) Enzymatic routes to enantiomerically pure aromatic a-hydroxy carboxylic
acids: a further example for the diversity of biocatalysis, Adv. Synth. Catal. 343 (6-7), 547–558
● Ress-Löschke, M., Friedrich, T., Hauer, B., Mattes, R., Engels, D., (2000) Method for producing
chiral carboxylic acids from nitriles with the assistance of a nitrilase or microorganisms which
contain a gene for the nitrilase, WO 00/23577, 2000 (DE 19848129 A1, 2000)
+
OH
CN
429