Industrial Biotransformations

EC 3.3 Acting on ether bonds
R 1 X R 2
H 2O
X = O or S
R 1,2 = organic residue
R 1 X H
EC 3.4 Acting on peptide bonds
R 1
O
R
N
H
2
H 2O
1,2
R = part of amino acids or proteins
R 1
O
OH
+ R 2 OH
+ R 2
H2N EC 3.5 Acting on carbon–nitrogen bonds other than peptide bonds
O
R 1 NR 2 H
H 2O
R1 = organic residue
R2 = hydrogen or organic residue
O
R 1 OH
+
R 2 NH2
2.2 Enzyme Classes
For some nitriles a similar reaction takes place. The enzyme involved is known as nitrilase
(EC 3.5.5.)
R C N
H 2O
R = aromatic, heterocyclic and certain unsaturated aliphatic residues
O
R OH
+
NH 3
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