Industrial Biotransformations

Penicillin acylase
Escherichia coli
4) Process parameters
yield: > 90 %
selectivity: > 95 %
ee: > 99 %
reactor type: repetitive batch (see flow schemes)
capacity: 2000 t · a –1 (worldwide)
down stream processing: filtration
company: DSM, The Netherlands
5) Product application
● The products are β-lactam antibiotics.
6) Literature
EC 3.5.1.11
● Bruggink, A. (1996) Biocatalysis and process integration in the synthesis of semi-synthetic
antibiotics, CHIMIA 50, 431–432
● Bruggink, A., Roos, E.C., de Vroom, E. (1998) Penicillin acylase in the industrial production of
β-lactam antibiotics, Org. Proc. Res. Dev. 2, 128–133
● Clausen, K. (1995) Method for the preparation of certain β-lactam antibiotics, Gist-Brocades N.
V., US 5,470,717
● Hernandez-Justiz, O., Fernandez-Lafuente, R., Terrini, M., Guisan, J.M. (1998) Use of aqueous
two-phase systems for in situ extraction of water soluble antibiotics during their synthesis by
enzymes immobilized on porous supports, Biotech. Bioeng. 59, 1, 73–79
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