Industrial Biotransformations

Styrene monooxygenase
Escherichia coli
1 = styrene
2 = (S)-styrene oxide
Fig. 1.14.13.69 – 1
1) Reaction conditions
[1]: 2 % (v/v organic phase)
[2]: 4.5 g · L –1 ·h –1 glucose (50 % w/v)
[3]: 1 % (v/v organic phase) n-octane
pH: 3.0
T: 30 °C
medium: two-phase system
reaction type: fed-batch
catalyst: whole cell
enzyme: styrene monooxygenase (StyA)
enzyme: reductase (StyB)
strain: Escherichia coli JM101(pSPZ10)
2) Remarks
O 2
NADPH,H +
1 2
EC 1.14.13.69
● Glucose is added continuously with a rate of 4.5 g · L –1 ·h –1 while the organic phase is added
batchwise one hour after starting the glucose feed.
● The host strain E.coli JM101 was transformed with a plasmid containing the styrene monooxygenase
from Pseudomonas sp. VLB120 and the alk regulatory system from P. oleovorans Gpo1.
● Bis(2-ethylhexyl)phthalate is used as an apolar carrier solvent. The organic phase also contains
2 % (v/v) n-octane, which is used as the inducer of the alk regulatory system.
● The system is limited by mass transfer of styrene from the organic to the aqueous phase.
E
● 2-Phenylethanol is produced as a by-product in approximately 10 % concentration due to the
ring opening reaction of the epoxide.
H 2O
NADP +
O
DSM
235