Industrial Biotransformations

d-Hydantoinase
Bacillus brevis
Fig. 3.5.2.2 – 2
● Racemic hydantoins are synthesized starting from phenol derivatives, glyoxylic acid and urea
via the Mannich condensation:
OH
+
O
H COOH
H2N NH2
● Re-use of cells through as immobilized cell catalyst is possible.
+
1 2
3
4
1 = phenol
2 = oxo-acetic acid
3 = urea
4 = 5-(4-hydroxy-phenyl)-imidazolidine-2,4-dione
Fig. 3.5.2.2 – 3
EC 3.5.2.2
● Several other companies have developed patented processes leading to d-hydroxyphenyl glycine
(Ajinomoto, DSM, Snamprogetti, Recordati and others, for example see page 415).
412
O
HO
HN
O
NH
O